Journal: Current Issues in Molecular Biology

Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

doi: 10.3390/cimb48030337

Figure Lengend Snippet: Effects of STG on the JAK2/STAT3 signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.

Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

Techniques: Expressing

Journal: Current Issues in Molecular Biology

Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

doi: 10.3390/cimb48030337

Figure Lengend Snippet: The effect of STG on osteogenic differentiation via the JAK2/STAT3 signaling pathway. ( A ) Expression of osteogenic marker proteins. ( B ) Statistical analysis of osteogenic marker protein expression. ( C ) ALP and ARS staining. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, ** p < 0.01; compared with the STG (+) AG490 (−) group, ### p < 0.001.

Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

Techniques: Expressing, Marker, Staining

Journal: Current Issues in Molecular Biology

Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

doi: 10.3390/cimb48030337

Figure Lengend Snippet: Schematic diagram of the role of STG in the JAK2/STAT3 pathway.

Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

Techniques: